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三倍体(3n)无籽西瓜组织培养快速育苗,是提高其繁殖系数的重要途径,近年来我们进行了这方面的研究,收到了一定效果. 一、材料和方法 参试材料有:“凤山一号”、“TA 6203” (均为北京市果林所提供)和“红樱”3个品种以及“奇美”无性系(自行建立的)。 (一)无性系的建立 3n西瓜种子经70％酒精泡5分钟,饱和漂白粉液泡2～3小时,无菌水冲洗3次,在无菌条件下取出种胚等处理过程后,再经0.1％升汞漂洗种胚10～15秒钟,无菌水冲洗4～5次后接种于培养基上。经过20天
Rapid breeding of triploid (3n) seedless watermelon tissue culture is an important way to improve its reproductive coefficient.In recent years, we conducted this research and received some results.Materials and Methods The materials of the experiment include: Mountain One “,” TA 6203 “(both provided by Beijing Fruit Grove) and” Hong Ying “three varieties and” Chi Mei "clones (self-established). (A) establishment of clones 3n watermelon seeds by 70% alcohol foam for 5 minutes, saturated bleach vacuoles 2 to 3 hours, rinsed with sterile water three times, under sterile conditions remove embryos and other treatment processes, and then 0.1% Rinsing Mercury rinse embryos 10 to 15 seconds, sterile water rinse 4-5 times after inoculation on the medium. After 20 days