【摘 要】
：
目的 研究细胞外信号调节蛋白激酶1/2(ERK1/2)信号通路对丹参制剂诱导大鼠骨髓基质细胞(BMSCs)向神经元样细胞分化过程的影响. 方法 分离、纯化大鼠BMSCs,取第3代细胞进行诱
论文部分内容阅读
目的 研究细胞外信号调节蛋白激酶1/2(ERK1/2)信号通路对丹参制剂诱导大鼠骨髓基质细胞(BMSCs)向神经元样细胞分化过程的影响. 方法 分离、纯化大鼠BMSCs,取第3代细胞进行诱导分化.根据诱导液不同分为空白对照组(A组)、丹参制剂组(B组)及PD98059(ERK1/2特异性抑制剂)组(C组).对各组细胞进行形态学观察,免疫组织化学方法检测神经元特异性烯醇化酶(NSE)、巢蛋白的蛋白表达,PCR检测NSE和巢蛋白的mRNA的表达. 结果 诱导1h后,A组细胞形态变化不明显;B组可见细胞形态发生改变,少数细胞呈锥形,细胞突起增多伸长,继续诱导培养5h后细胞突起增多,呈类神经元细胞分化,突起间相互交织;C组细胞总数、类神经元细胞和突起数目均较B组减少.诱导5h后,B组NSE和巢蛋白染色细胞阳性率和mRNA表达量较A、C组明显增高,差异均有统计学意义(P＜0.05). 结论 丹参制剂可以促进BMSCs向神经元样细胞分化,ERK 1/2的特异性抑制剂PD98059能够拮抗丹参制剂促分化作用,提示ERK1/2信号通路可能参与了丹参制剂诱导BMSCs向神经元样细胞分化的过程.
其他文献
The present study investigated whether an irrigation system could be established to save water and increase grain yield to enhance water productivity by proper
In C3 plants, carbon isotope discrimination (△) has been proposed as an indirect selection criterion for grain yield. Reported correlations between △ and grai
Bio-water saving is to increase water use efficiency of crops or crop yield per unit of water input. Plant water use efficiency is determined by photosynthesis
采用二乙基氨基乙基52和葡聚糖凝胶G100柱层析法,从甘薯中分离纯化甘薯糖蛋白,并对其进行结构分析.用SDS-聚丙烯酰胺凝胶电泳检验纯度和分子质量;根据β-消除反应判断糖肽键
The carbon Isotope discrimination (δ13C) of leaves has been shown to be correlated with the transpiration efficiency of leaves in a wide range of species. This
Two upland rice varieties (IRAT109, IAPAR9) and one lowland rice variety (Zhenshan 97B) were planted in summer and treated with both normal (full water) and dro
Water shortage is increasingly limiting the luxury use of water in rice cultivation. In this study, non-flooded mulching cultivation of rice only consumed a fra
不同指标值的规范化是使用Vague集综合评价的第一步.针对现有算法的不足,根据Vague集理论,提出了效益型、成本型、固定型、区间型、偏离型、偏离区间型等六种类型单值指标Vag
Cre/lox P系统由Cre位点特异重组酶和可被Cre特异性识别的lox P位点构成,该系统广泛用于条件性基因敲除和表达,以研究基因功能.为了表达和纯化一种细胞可透过性Cre重组酶(即H
近年来,促进受损脑细胞再生成为神经系统退行性病变治疗研究的重要方向.研究人员越来越重视脑细胞损伤后的自我修复[1],对脑细胞损伤后自我修复机制和促进细胞再生的治疗手段