Establishment and Application of a Real-time Fluorescent Quantitative PCR Method for Detection of Po

• 作 者: Dong Lin Wei Feng Guan Yu Liu
• 机 构: CollegeofAnimalScienceandVeterinaryMedicine,ShandongBinzhouAnimalScienceandVeterinaryMedicineAcademy
• 出 处: 动物与饲料科学：英文版
• 发表日期: 2015年4期
• 关键词: 荧光定量聚合酶链反应 猪圆环病毒2型 PCR方法 检测 猪繁殖与呼吸综合征病毒 实时 Green DNA模板 Porcine circovirus type 2
• 基金项目: Supported by Shandong Province Natural Science Fund Project.

[Objective]To establish a real-time fluorescent quantitative polymerase chain reaction(PCR) method with SYBR Green I for the detection of porcine circovirus type 2(PCV2).[Methods]Specific primers were designed to amplify the conserved gene segments of PCV