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本研究利用热处理和Sephadex G-150凝胶过滤层析等方法,从原发性高血压病患者(EHS)红细胞中部分纯化了抗高血压因子(AHF)。AHF具有热稳定性,分子量大于6kDa;能明显降低卒中易感型自发性高血压大鼠(SHR_(sp))血压,腹腔一次注入AHF(1.6mg/kg)30min后,SHR_(sp)收缩压从原来的27.6±0.7kPa降低到21.4±0.8kPa(p<0.001),4h后收缩压恢复至原水平。AHF能显著抑制自发性高血压大鼠(SHR)和肾性高血压大鼠(RHR)主动脉(A)及肠系膜动脉(MA)血管平滑肌(VSM)Ca~(2+)内流。且对MA Ca~(2+)内流的抑制作用强于A。以上结果表明:EHS红细胞中存在AHF,能显著降低高血压大鼠血压,其降压机制可能与其抑制VSM特别是小动脉VSM Ca~(2+)内流有关。
In this study, anti-hypertensive factors (AHFs) were partially purified from EHs red blood cells by heat treatment and Sephadex G-150 gel filtration chromatography. AHF has a thermal stability of more than 6 kDa; it can significantly reduce the blood pressure of stroke-prone spontaneously hypertensive rats (SHR-sp) and the SHR sp systolic pressure From 27.6 ± 0.7kPa to 21.4 ± 0.8kPa (p <0.001), systolic blood pressure returned to the original level after 4h. AHF could significantly inhibit the Ca ~ (2+) influx in the aorta of the spontaneously hypertensive rats (SHR) and the renal hypertensive rats (RHR) and the vascular smooth muscle (VSM) of the mesenteric artery (MA) And the inhibitory effect on MA Ca 2+ influx was stronger than A. The above results indicate that the presence of AHF in erythrocytes of EHS can significantly reduce the blood pressure in hypertensive rats, and its antihypertensive mechanism may be related to its inhibition of the influx of VSM, especially the arteriolar VSM Ca ~ (2+).