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为研究肝脏MED1对脂质代谢的影响,以肝脏MED1特异性敲除(MED1ΔLiv)小鼠为模型,对其进行基因型鉴定,H&E染色观察肝脏组织学变化,免疫组织化学染色检测肝脏MED1蛋白表达;高脂饲料(脂肪含量为60%)饲喂小鼠,并分别在0、1、2和4周动态检测血浆胆固醇和甘油三酯及血糖水平.结果显示,与MED1fl/fl小鼠相比,MED1ΔLiv小鼠仅肝脏MED1 mRNA表达水平显著降低,其它组织表达无明显变化.高脂饲喂1周和2周,MED1ΔLiv小鼠血浆总胆固醇水平显著升高(P<0.01);普通或高脂饲料饲喂状态下,与MED1fl/fl小鼠相比,MED1ΔLiv小鼠血糖水平均显著降低(P<0.05).短期给予高脂饲料可诱导MED1ΔLiv小鼠呈现高胆固醇血症,提示MED1在胆固醇代谢中发挥重要调控作用.
In order to study the effect of liver MED1 on lipid metabolism, the liver MED1-specific knockout (MED1ΔLiv) mice were used as the model to identify the genotype. H & E staining was used to observe the histological changes of the liver. Immunohistochemical staining was used to detect the expression of MED1 Mice were fed with high-fat diet (fat content 60%), and plasma cholesterol, triglyceride and blood glucose levels were dynamically measured at 0, 1, 2, and 4 weeks respectively.The results showed that compared with MED1fl / fl mice , MED1ΔLiv mice only had significantly lower expression of MED1 mRNA in liver and no significant changes in other tissues.Plasma total cholesterol levels were significantly increased in MED1ΔLiv mice at 1 week and 2 weeks after feeding with high fat diet (P <0.01) Compared with MED1fl / fl mice, the blood glucose levels of MED1ΔLiv mice were significantly decreased (P <0.05), and the short-term high fat diet induced hypercholesterolemia in MED1ΔLiv mice, suggesting that MED1 may play an important role in cholesterol metabolism Play an important role in regulation and control.