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采用35个不同来源的稻瘟病菌菌株,对3个稻瘟病抗性基因Pi9、Pi2、Pizt的供体亲本和以日本晴为遗传背景的转基因近等基因系进行室内接种鉴定,比较分析3个基因的抗谱差异。结果表明:Pi9和Pi2的抗谱较广,抗性频率均达到94.2%,Pi9对菌株ROR1和X2007A–7表现感病,Pi2对菌株CHNOS60–2–3和RB14表现感病,Pizt抗谱较窄,抗性频率仅为60%,使用13对SSR引物分析其中20个稻瘟病菌菌株的遗传多样性,可划分为4个宗谱,菌株的遗传宗谱和来源相关性不显著,与致病性有一定的相关性。
35 isolates of Magnaporthe grisea were used for inoculation of three rice blast resistance genes Pi9, Pi2 and Pizt and the near-isogenic lines with Nipponbare as the genetic background. Three genes Anti-spectrum differences. The results showed that the resistant spectrum of Pi9 and Pi2 was wide and the frequency of resistance was up to 94.2%. Pi9 showed susceptible to strains ROR1 and X2007A-7, Pi2 showed susceptible to strains CHNOS60-2-3 and RB14, The frequency of resistance was only 60%. The genetic diversity of 20 strains of Magnaporthe grisea was analyzed using 13 pairs of SSR primers, which could be divided into four genealogies. The genetic lineage of the strains was not significantly correlated with the source Disease has a certain relevance.