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目的探讨肺缺血-再灌注损伤(PIRI)时Fas/FasL蛋白表达的变化及葛根素(Pue)的干预。方法采用在体兔单肺原位PIRI模型。实验兔90只,随机分为假手术对照组(sham,30只)、PIRI组(30只)和Pue组(30只)。每组又分为再灌注1,3,5 h 3个亚组,每个亚组10只,分别于再灌注1,3,5 h 3个时间点取左肺组织,观察Fas/FasL蛋白的表达、凋亡指数(AI)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果PIRI 1、3、5 h,Pue组Fas/FasL mRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达,明显低于同一时间点PIRI组(P<0.05);AI和IQA值显著低于PIRI组(P<0.01和P<0.05);肺组织形态学异常改变不同程度减轻。结论Pue可下调肺组织Fas/FasL蛋白的表达而减轻细胞凋亡,对PIRI发挥积极的防治作用。
Objective To investigate the changes of Fas/FasL protein expression in pulmonary ischemia-reperfusion injury (PIRI) and the intervention of Puerarin. Methods The rabbit lung in situ PIRI model was used. The experimental rabbits were randomly divided into sham-operated control group (sham, 30), PIRI group (30) and Pue group (30). Each group was divided into three subgroups: 1, 3, and 5 h after reperfusion. Each subgroup was divided into 10 groups. Left lung tissue was taken at 1, 3, and 5 h after reperfusion to observe the expression of Fas/FasL protein. Expression, apoptotic index (AI), quantitative assessment index (IQA) of lung injury, and histological changes under light and electron microscope. Results In PIRI 1, 3, and 5 h, Fas/FasL mRNA expression in Pue group was weakly positive in the pulmonary arteriole (outer) membrane, pulmonary intima, alveolar epithelium, and lung bronchial epithelium, which was significantly lower than the PIRI group at the same time point. (P <0.05); AI and IQA values were significantly lower than PIRI group (P <0.01 and P <0.05); abnormal changes of lung tissue morphology were alleviated to varying degrees. Conclusion Pue can down-regulate the expression of Fas/FasL protein in lung tissue and reduce apoptosis, and it plays an active role in prevention and treatment of PIRI.