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目的 :观察人参皂甙 Rh2 对 C6胶质瘤细胞的增殖抑制和诱导凋亡作用 ,并在分子水平探讨其作用机理。方法 :细胞计数法检测细胞增殖 ,流式细胞仪分析细胞周期和凋亡。结果 :1 Rh2 2 ,4,8μmol/L对 C6胶质瘤细胞有增殖抑制作用 ,且呈明显量效关系 ;2 Rh2 作用 72 h后 ,4μmol/L浓度对 C6胶质瘤细胞诱导凋亡作用最为明显 ;3 Rh2 作用后 ,G0 /G1期细胞百分率明显下降 ,而S期细胞百分率显著增加。结论 :Rh2 对 C6胶质瘤细胞有明显的增殖抑制和诱导凋亡作用 ,且这种作用有周期特异性。
Objective: To observe the effect of ginsenoside Rh2 on proliferation inhibition and apoptosis induction of C6 glioma cells, and to explore its mechanism of action at the molecular level. Methods: Cell proliferation was detected by cell counting, and cell cycle and apoptosis were analyzed by flow cytometry. RESULTS: 1 Rh2 2, 4 and 8 μmol/L inhibited the proliferation of C6 glioma cells in a dose-dependent manner. 2 After 72 h of Rh2 treatment, the concentration of 4 μmol/L induced apoptosis in C6 glioma cells. The most obvious; 3 Rh2 effect, the G0 / G1 phase percentage of cells decreased significantly, while the percentage of S phase cells increased significantly. Conclusion : Rh2 can significantly inhibit proliferation and induce apoptosis of C6 glioma cells, and this effect is cycle-specific.