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目的:研究大孔吸附树脂分离纯化桂花总黄酮工艺条件,为桂花总黄酮的工业化生产提供实验依据。方法:以贵州产桂花为原料,以桂花总黄酮吸附量及回收率等为考察指标,选用AB-8型大孔吸附树脂对桂花总黄酮进行分离纯化,分别采用静态试验、动态试验等考察AB-8型大孔树脂对桂花总黄酮的分离纯化最佳工艺条件及效果。结果:pH值、洗脱剂、温度、上柱液浓度、径高比、流速、总黄酮与树脂质量比等工艺条件对桂花总黄酮的吸附洗脱量、回收率等影响甚大。结论:AB-8型大孔树脂分离纯化桂花总黄酮最佳工艺条件为:上柱液pH4～ 5;洗脱剂为70%乙醇,洗脱剂用量为4倍树脂体积,流速3～ 4 mL/min;上柱总黄酮质量与树脂质量比为1:9.4,上柱液总黄酮浓度为17.86 mg/mL,流速2～ 3 mL/min;冲洗杂质用水体积2～ 3 BV,流速2～ 3mL/min;径高比1.5/21.6;温度升高,吸附量下降但洗脱率加大。
Objective: To study the conditions for the separation and purification of total flavonoids from Osmanthus fragrans with macroporous adsorption resin, and to provide an experimental basis for the industrial production of total flavonoids from Osmanthus fragrans. Methods: Osmanthus fragrans flowers from Guizhou were used as raw materials. The adsorption and recovery of total flavonoids in Osmanthus fragrans were investigated. The total flavonoids of Osmanthus fragrans were isolated and purified by AB-8 macroporous adsorption resin. The static and dynamic tests were used to study the effects of AB Separation and Purification of Total Flavonoids from Osmanthus fragrans - 8 Macroporous. Results: The conditions such as pH value, eluent, temperature, concentration on the column, diameter-height ratio, flow rate and the ratio of total flavonoids to resin mass ratio had great influence on the adsorption and elution amount of the total flavonoids in Osmanthus fragrans. CONCLUSION: The optimal conditions for the separation and purification of the total flavonoids from Osmanthus fragrans with AB-8 macroporous resin are as follows: the upper column liquid is pH 4-5; the eluent is 70% ethanol; the eluent is 4 times the volume of resin; the flow rate is 3-4 mL / min; the mass ratio of the total flavonoids to resin on the column was 1: 9.4, the total flavonoid concentration on the column was 17.86 mg / mL and the flow rate was 2-3 mL / min; the volume of flushing water was 2-3 BV and the flow rate was 2-3 mL / min; diameter ratio of 1.5 / 21.6; the temperature increased, the adsorption decreased but the elution rate increased.