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从1例未免疫雏番鸭细小病毒活疫苗与鹅细小病毒活疫苗的临床病死雏番鸭脏器中成功分离到1株番鸭细小病毒(FJM3株)。为明确其基因组特征,运用PCR方法,扩增出番鸭细小病毒FJM3株全基因组。结果表明,FJM3株基因组全长为5017nt,其基因组结构和经典番鸭细小病毒参考毒株一致。序列比对结果表明,FJM3株全基因组序列与GenBank中登录的经典MDPV参考株(FM株)核苷酸序列同源性为94.0%,与番鸭源GPV参考株(Y株)核苷酸序列同源性为85.6%。全基因组遗传进化分析表明,FJM3株处于MDPV遗传进化分支;VP1基因遗传分析表明,FJM3株处于经典MDPV遗传进化分支;VP3遗传进化表明,FJM3株处于GPV遗传进化分支。对FJM3株和参考株(FM株、Y株和SYG61v)进行遗传重组分析表明,该毒株于存在2处MDPV与GPV的基因重组现象。
One Muscovy duck parvovirus (FJM3 strain) was successfully isolated from the diseased young Muscovy ducklings of one unvaccinated live Muscovy duck parvovirus live vaccine and goose parvovirus live vaccine. In order to clarify its genomic characteristics, the whole genome of Muscovy duck parvovirus FJM3 was amplified by PCR. The results showed that the full-length genome of FJM3 strain was 5017nt and its genome structure was consistent with the reference strain of classical Muscovy duck parvovirus. Sequence alignment showed that the nucleotide sequence of the FJM3 strain was 94.0% identical to that of the classical MDPV reference strain (FM strain) registered in GenBank, which was identical to the nucleotide sequence of Muscovy duck GPV reference strain (Y strain) Homology was 85.6%. Genome-wide phylogenetic analysis showed that FJM3 was in the MDPV genetic branch. The genetic analysis of VP1 gene showed that FJM3 was in the classical MDPV branch of genetic evolution. The genetic evolution of VP3 indicated that FJM3 was in the branch of genetic evolution of GPV. Genetic recombination analysis of FJM3 and reference strains (FM strain, Y strain and SYG61v) showed that there were two gene recombination phenomena in MDPV and GPV.