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目的研究核苷酸切除修复交叉互补基因1(ERCC1)的SNP基因型和慢性苯中毒遗传易感性的关联,为建立慢性苯中毒易感人群的生物标志及其防治研究提供理论依据。方法采集病例组102名慢性苯中毒患者、对照组204名人员静脉血样,应用PCR法检测ERCC1Asn118Asn(rs11615)、C8092A(rs3212986)SNP位点基因型,分析基因多态位点和慢性苯中毒易感性是否有关,以比值比(odds ratio,OR)及其95%可信限(95%confidence interval,95%CI)表示关联强度。结果 ERCC1 Asn118Asn(rs11615)TT基因型可使慢性苯中毒发生风险增高(ORadj=3.251,95%CI=1.365~7.743,χ2=6.718,P=0.010),未发现ERCC1 C8092A(rs3212986)等位点与慢性苯中毒发生的关联。结论在相同的苯作业暴露环境下,携带ERCC1Asn118Asn(rs11615)TT基因型的个体发生慢性苯中毒的风险增高,ERCC1Asn118Asn(rs11615)多态可能作为慢性苯中毒发病危险性增加的生物学标志之一。
Objective To study the relationship between SNP genotypes of nucleotide excision repair cross-complementary gene 1 (ERCC1) and susceptibility to chronic benzene poisoning, and to provide a theoretical basis for the establishment of biomarkers for prevention and treatment of chronic benzene poisoning susceptible population. Methods A total of 102 patients with chronic benzene poisoning and 204 control subjects were enrolled in this study. Genotypes of ERCC1Asn118Asn (rs11615) and C8092A (rs3212986) SNPs were detected by PCR. The genetic polymorphisms and susceptibility to chronic benzene poisoning Whether related, odds ratio (OR) and 95% confidence interval (95% confidence interval, 95% CI) said correlation strength. Results The TT genotype of ERCC1 Asn118Asn (rs11615) increased the risk of chronic benzene poisoning (ORadj = 3.251, 95% CI = 1.365-7.743, χ2 = 6.718, P = 0.010). No association was found between ERCC1 C8092A (rs3212986) The association of chronic benzene poisoning. Conclusion The risk of chronic benzene poisoning in individuals with ER genotype of ERCC1Asn118Asn (rs11615) is increased under the same benzene exposures. ERCC1Asn118Asn (rs11615) polymorphism may be one of the biomarkers of increased risk of chronic benzene poisoning.