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英国药典记载测定透明质酸酶的方法是预先使之形成粘蛋白凝块,这方法很不精确。作者发现,透明质酸酶水溶液的紫外吸收光谱在280nm处呈现一最大特征吸收峰,浓度范围于100～900μg/ml时符合比尔定律。本文报道的分光光度测定法,既精确又简便。分析方法:将已秤重的一颗栓剂于50毫升圆锥烧瓶中,加12.5ml水,水浴上加热至基质熔化后,小心搅和5分钟并防止乳化。用冰块冷却烧瓶,当脂肪凝聚后,用蒸馏水湿润过的褶叠滤纸将水层滤至50毫升容量瓶中。加12.5ml水至烧瓶内的残留物中,重复以上操作,滤液加水至刻度。所得溶液以蒸馏水作对照,于1cm比色池中在280nm处测定吸收度。
British Pharmacopoeia records hyaluronidase method is pre-made to form a mucin clot, this method is not accurate. The authors found that the UV absorption spectra of aqueous hyaluronidase showed a maximum characteristic absorption peak at 280 nm and Beer’s law was obeyed at a concentration range of 100-900 μg / ml. The spectrophotometry reported in this paper is both accurate and easy. Method of analysis: A weighed suppository was placed in a 50 ml conical flask, and 12.5 ml of water was added. The mixture was heated in a water bath until the matrix melted. Carefully stir for 5 minutes and prevent emulsification. Cool the flask with ice and when the fat has condensed, filter the aqueous layer in a 50 ml volumetric flask with pleated filter paper moistened with distilled water. Add 12.5ml of water to the residue in the flask, repeat the above operation, the filtrate was added water to the mark. The resulting solution was compared with distilled water and the absorbance was measured at 280 nm in a 1 cm cuvette.