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目的探讨在有无慢性乙型肝炎的不同背景下p53基因第72密码子多态性(R72P)与中国人肝细胞癌(hepatocellular carcinoma,HCC)遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态方法,检测469例HCC(HBsAg阴性110例、HBsAg阳性359例)与567名对照(HBsAg阴性430例、HBsAg阳性137例)的p53R72P基因型分布及差异。结果全样本以及HBsAg阳性样本的HCC与对照间的基因型分布差异均无统计学意义。但在HBsAg阴性人群中,72P是HCC发生的危险因素(OR=1.69,95%CI=1·25~2.27)。与R/R基因型相比,R/P的HCC风险增加至1.73倍(95%CI=0.96~3.11),P/P的HCC风险显著增加至3.29倍(95%CI=1.58~6.86)。携带72P的男性个体、HCC家族史阳性个体的HCC风险分别进一步增加至9.39倍(95%CI=3.08~28.62)和11.14倍(95%CI=1.62~76.67)。结论p5372P增加HBsAg阴性中国人的HCC风险,并与男性、HCC家族史在增加HCC风险中有协同作用。
Objective To investigate the relationship between the polymorphism of the 72nd codon of p53 (R72P) and the genetic susceptibility to hepatocellular carcinoma (HCC) in China with and without chronic hepatitis B (CHB). Methods The genotypes of p53R72P in 469 HCC patients (110 HBsAg negative, 359 HBsAg positive) and 567 controls (HBsAg negative 430, HBsAg positive 137) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) And differences. Results There was no significant difference in genotype distribution between HCC and controls in all samples and HBsAg positive samples. However, in HBsAg-negative population, 72P is a risk factor for HCC (OR = 1.69, 95% CI = 1.25-2.27). Compared with R / R genotype, R / P increased the risk of HCC to 1.73 times (95% CI = 0.96 ~ 3.11) and P / P increased HCC risk to 3.29 times (95% CI = 1.58-6.86). The risk of HCC in individuals with positive HCC family members was further increased to 9.39-fold (95% CI = 3.08-28.62) and 11.14-fold (95% CI = 1.62-76.67) in men with 72P, respectively. Conclusion p5372P increases the risk of HCC in HBsAg-negative Chinese and has a synergistic effect with male and HCC family history in increasing the risk of HCC.