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[目的]探讨不同产地、不同海拔红景天药材中的红景天苷含量.[方法]采用Kromasil C18柱(150.0mm×4.6mm,5μm);流动相以甲醇-水(15∶85),检测波长为275 nm,流动速度为1 mL/min,柱温为30℃,进样量为10μL.[结果]红景天苷在24.8～124.0 mg/L范围内线性关系良好,回归方程为Y=1.265×104 X+6.847×103,R=0.999 7,平均回收率为98.86%,RSD为1.38%(n=5).不同产地、不同海拔红景天药材中的红景天苷含量间有差异.[结论]红景天苷含量与产地、海拔及野生或栽培有一定的关系.
[Objective] To investigate the content of salidroside in Rhodiola rosea from different producing areas and different altitudes. [Methods] Kromasil C18 column (150.0mm × 4.6mm, 5μm) was used. The mobile phase consisted of methanol-water (15:85) The detection wavelength was 275 nm, the flow rate was 1 mL / min, the column temperature was 30 ℃, and the injection volume was 10 μL. [Result] Salidroside had a good linearity in the range of 24.8 ~ 124.0 mg / L with a regression equation of Y = 1.265 × 104 X + 6.847 × 103, R = 0.999 7, the average recovery was 98.86% and the RSD was 1.38% (n = 5) .The content of salidroside in Rhodiola rosea Differences. [Conclusion] The content of salidroside has some relationship with the origin, elevation and wild or cultivation.