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以周豆12为材料,研究了不同浓度的抗坏血酸(ASA)对盐胁迫下大豆愈伤组织生长的影响,以期为大豆抗盐胁迫研究提供参考。结果表明:盐胁迫下,大豆愈伤组织的相对含水量、相对干重增长速率、可溶性蛋白含量显著降低,Pro、MDA含量以及SOD、POD活性显著增加(P<0.01);施用ASA显著提高了盐胁迫下愈伤组织的相对含水量、相对干重增长速率、可溶性蛋白和Pro含量,SOD和POD活性也显著升高,膜脂过氧化产物MDA含量显著降低(P<0.01)。由此可见,ASA可以通过提高大豆愈伤组织的相对含水量、可溶性蛋白和Pro含量以及SOD、POD活性来维持细胞膜的稳定性,降低膜脂过氧化伤害程度,从而缓解盐胁迫对愈伤组织生长的抑制,并以8 mmol·L-1外源ASA缓解效应最好。
The effects of different concentrations of ascorbic acid (ASA) on the growth of soybean callus under salt stress were studied using Zhouou 12 as a material, in order to provide a reference for the study on the salt tolerance of soybean. The results showed that under salt stress, the relative water content, relative dry weight growth rate, soluble protein content, Pro and MDA contents and the activities of SOD and POD in soybean callus were significantly increased (P <0.01) Under salt stress, the relative water content, relative dry weight growth rate, soluble protein and Pro content, SOD and POD activities, and MDA content in membrane lipid peroxidation products significantly decreased (P <0.01). Thus, ASA could improve the stability of cell membrane and increase the damage of membrane lipid peroxidation by increasing the relative water content, soluble protein, Pro content and the activities of SOD and POD in soybean callus to alleviate the effects of salt stress on callus Growth inhibition, and exogenous ASA 8 mmol · L-1 best mitigation effect.