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目的：考察将转化生长因子β（ＴＧＦ－β）、胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）、碱性成纤维细胞生长因子（ｂＦＧＦ）及血小板衍生性生长因子（ＰＤＧＦ）４种生长因子两两交互应用对人胚成骨样细胞增殖与分化的影响。方法：在不同的实验间期检测成骨样细胞３Ｈ－胸腺嘧啶脱氧核苷、３Ｈ－脯氨酸的掺入量和碱性磷酸酶的含量。结果：ＰＤＧＦ、ｂＦＧＦ、ＩＧＦ－Ⅱ和ＴＧＦ－β４种生长因子交互联合应用，均对促进体外培养的人胚成骨样细胞的增殖和分化功能具有协同作用。结论：具有协同作用的生长因子联合应用，可提高其促进骨形成的生物效应。
OBJECTIVE: To investigate the effect of transforming growth factor-β (TGF-β), insulin-like growth factor Ⅱ (IGF-Ⅱ), basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) Effect of Interaction on Proliferation and Differentiation of Human Embryogenesis. Methods: 3H-thymidine, 3H-proline incorporation and alkaline phosphatase in osteoblast-like cells were detected at different experimental intervals. RESULTS: The combination of four growth factors, PDGF, bFGF, IGF-Ⅱ and TGF-β, had synergistic effects on the proliferation and differentiation of human osteoblast-like cells in vitro. Conclusion: Combined application of growth factors with synergistic effect can improve the biological effect of promoting bone formation.