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目的分析脂联素基因(apM1)多态性与代谢综合征的相关性。方法无亲缘关系的344例汉族不同程度代谢异常者,根据代谢异常程度分为代谢综合征组180例(MS组)与代谢异常组164例,选择同期50例无代谢异常者为对照组,采用多聚酶链反应-限制性片段长度多态性方法检测3组apM1基因外显子2+45和外显子3+331 2个位点的基因多态性,并检测血脂等指标。结果中国汉族人apM1基因外显子2+45位点存在基因多态性,有TT,TG,GG 3种基因型;3组间基因型频率及等位基因频率比较差异无统计学意义(P>0.05);G等位基因携带者(G+)与非G等位基因携带者(G-)血清脂联素、血脂、血糖及胰岛素水平差异无统计学意义(P>0.05);中国汉族人外显子3+331位点不存在基因多态性,均为TT基因型。结论中国汉族人脂联素基因外显子2+45位点的多态性与代谢综合征无相关性;外显子3+331位点不存在基因多态性。
Objective To analyze the association between adiponectin gene (apM1) polymorphism and metabolic syndrome. Methods There were 344 Han unrelated patients with different degrees of metabolic abnormalities. According to the degree of metabolic abnormalities, they were divided into metabolic syndrome group (180 cases) and metabolic abnormal group (164 cases). Fifty cases without metabolic abnormalities were selected as the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the gene polymorphism of exon 2 +45 and exon 3 + 331 in three groups of apM1 gene. Results There were 3 genotypes of TT, TG and GG in exon 2 + 45 of apM1 gene in Han Chinese. There was no significant difference in genotype frequency and allele frequency between the three groups (P > 0.05). There was no significant difference in serum adiponectin, serum lipids, blood glucose and insulin between carriers of G allele (G +) and non-G allele carriers (G-) (P> 0.05) Exon 3 + 331 loci do not exist gene polymorphisms, are TT genotypes. Conclusion There is no association between the polymorphism of exon 2 + 45 locus in Chinese Han population and metabolic syndrome. There is no polymorphism in exon 3 + 331 locus.