论文部分内容阅读
通过丁酰壳聚糖与丙烯酰氧基丙基二甲氧基硅烷的迈克尔加成反应合成了含有甲氧硅烷基 (Methoxysilyl )的丁酰壳聚糖 (Mos butyrylchitosan) ,Mos基团通过水解缩合成树枝网络膜 (DBCSF)。SEM测定表明 ,该膜具有微孔结构。当丁酰壳聚糖树枝网络膜固定辣根过氧化酶 (HRP)修饰金电极表面 ,采用示差脉冲极谱 (DPV )方法对过氧化氢进行监测 ,测量限度可达到 2× 10 -10 mol/L ,传感器 4℃储存 2个月以后酶的活性可保持 75 %。
Methyl butyryl chitosan (Mos butyrylchitosan) containing methoxysilyl was synthesized by Michael addition of butyryl chitosan and acryloxypropyldimethoxysilane. The Mos group was hydrolyzed Synthetic branch network membrane (DBCSF). SEM measurements show that the membrane has a microporous structure. Hydrogen peroxide was monitored by Differential Pulse Polarography (DPV) when butyrylchitosan network was immobilized on horseradish peroxidase (HRP) -based electrode. The limit of detection was up to 2 × 10 -10 mol / L, the enzyme stored at 4 ℃ for 2 months after the enzyme activity can be maintained at 75%.