论文部分内容阅读
以5种野生猕猴桃为试验材料,采用DNA条形码技术,对6个DNA条形码标记rbcL、matK、psbA、trnL-F、ITS、trnH-psbA进行序列分析,筛选出能鉴别猕猴桃种间分子差异的DNA条形码,以期利用DNA条形码技术用于猕猴桃育种。结果表明:通过序列碱基含量分析,trnL-F和ITS条形码序列的GC含量最高,为51.1%~55.2%,trnH-psbA的GC含量最小,约为32.0%;通过Blast比对,结果显示5个猕猴桃野生种与基因库中已登录种类的6个DNA条形码相似度在99%以上,而有些与基因库中已登录种类有所差异,体现出猕猴桃种内的遗传多样性或种间存在基因渗透;从系统进化树结果分析显示,ITS标记能将上述5个野生种明显区分,基因进化多样性两两比对分析显示,5个猕猴桃野生种之间差异明显;Tajima’s中性检验中,trnL-F,ITS和matK具有较高的核苷酸多样性和中性检验值。研究比较了6种DNA条形码标记,认为ITS种间多样性较高,差异明显,较适宜作为猕猴桃DNA条形码。
Five wild kiwifruits were used as experimental materials, and DNA barcoding technique was used to analyze the sequence of 6 barcoded rbcL, matK, psbA, trnL-F, ITS and trnH-psbA. Barcodes, with a view to using DNA barcoding technology for kiwi breeding. The results showed that GC content of trnL-F and ITS barcode sequences was the highest (51.1% -55.2%), and the content of trnH-psbA was the lowest (about 32.0%) by sequence base analysis. By Blast comparison, the results showed that 5 The similarity of 6 DNA barcodes between kiwifruit wild type and the registered species in the gene bank is over 99%, while some are different from those registered in the gene bank, indicating the genetic diversity within kiwifruit or the gene The results of phylogenetic tree analysis showed that the ITS markers could clearly distinguish the above 5 wild species, and the pairwise alignment analysis showed that there were significant differences among 5 wild kiwifruit species. In Tajima’s neutrality test, trnL -F, ITS and matK have high nucleotide diversity and neutrality test value. Six kinds of DNA barcoding markers were compared and studied. The diversity of ITS species was high and the difference was obvious, which was more suitable for kiwifruit DNA barcode.