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目的:比较两类三聚氰胺分子印迹聚合物的性能及应用。方法:以三聚氰胺为模板分子,分别以α-甲基丙烯酸和丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,60℃聚合24 h,制备了两种分子印迹聚合物。比较研究了它们对三聚氰胺的特异识别作用和氢键作用力大小;同时研究了印迹聚合物固相萃取柱的应用。结果:研究发现,两类分子印迹聚合物对三聚氰胺都存在一类等价结合位点,都具有特异性的识别作用;用α-甲基丙烯酸单体合成的印迹聚合物对模板分子的氢键作用力和结合能力更强;用该分子印迹聚合物作固相萃取柱,其液相色谱检测回收率>90%。对奶粉和液态奶提取液的加标回收率分别为90.4%~95.8%、91.5%~92.7%。结论:自制印迹聚合物固相萃取柱对牛奶样品预处理,试剂用量少,萃取回收时间短,检测牛奶样品中三聚氰胺,结果满意。
Objective: To compare the properties and applications of two types of melamine molecularly imprinted polymers. Methods: Using melamine as the template molecule, α-methacrylic acid and acrylamide as functional monomers, ethylene glycol dimethacrylate as crosslinking agent, azobisisobutyronitrile as initiator and polymerization at 60 ℃ for 24 h, two molecularly imprinted polymers were prepared. Their specific recognition of melamine and the size of hydrogen bonding force were studied comparatively. The application of imprinted polymer solid phase extraction column was also studied. Results: It was found that both types of molecularly imprinted polymers have a class of equivalent binding sites to melamine, which have specific recognition. The hydrogen bonding of the template molecule with the imprinted polymer synthesized with α-methacrylic monomer Stronger binding force and binding capacity. The molecularly imprinted polymer was used as solid-phase extraction column and the recovery rate of liquid chromatography was> 90%. The spiked recoveries of milk powder and liquid milk extract were 90.4% -95.8% and 91.5% -92.7%, respectively. CONCLUSION: Pretreatment of milk sample with homemade imprinted polymer solid phase extraction column with less reagent, shorter extraction and recovery time, and satisfactory detection of melamine in milk samples.