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在盆栽条件下研究了ABA和6-BA对水分胁迫下玉米幼苗中一些碳素同化关键酶的影响。结果表明,水分胁迫过程中玉米幼苗的1,5-二磷酸核酮糖羧化酶(RuBPC)活性先升后降,磷酸烯醇式丙酮酸羧化酶(PEPC)和丙酮酸磷酸二激酶(PPDK)活性呈降低趋势。叶面喷施10~(-5)mol/L脱落酸(ABA)在未水分胁迫时对其RuBPC、PEPC和PPDK活性稍有促进作用,干旱后则抑制RuBPC活性,而对PEPC和PPDK活性影响不大。喷施同样浓度的6-苄氨基嘌呤(6-BA)则显著促进水分胁迫条件下玉米幼苗3种酶活性。ABA或6-BA对水培玉米幼苗RuBPC、PEPC及PPDK活性的促进作用可被环已正胺(CHX)强烈抑制,而ABA、6-BA或CHX对萃取的酶液无直接影响,表明ABA或6-BA的作用可能与促进上述酶类的合成有关。结合叶水势、气孔阻力、叶绿素含量和光合速率等的变化,可以看出ABA或6-BA均可提高水分胁迫条件下玉米幼苗的光合作用,从而提高其抗旱性,但二者的作用方式不同。
The effects of ABA and 6-BA on some carbon assimilation key enzymes in maize seedlings under water stress were studied under pot culture. The results showed that RuBPC activity of maize seedlings increased first and then decreased under water stress. The activities of phosphoenolpyruvate carboxylase (PEPC) and pyruvate phosphate dikinase PPDK) activity decreased. Foliar application of 10 ~ (-5) mol / L abscisic acid (ABA) promoted its activities of RuBPC, PEPC and PPDK slightly under the condition of no water stress, but inhibited the activity of RuBPC after drought, while the activity of PEPC and PPDK Not big. The same concentration of 6-benzylaminopurine (6-BA) significantly promoted three enzyme activities of corn seedlings under water stress. ABA or 6-BA could significantly inhibit the activities of RuBPC, PEPC and PPDK in hydroponic maize seedlings. However, ABA, 6-BA or CHX had no direct effect on the extracted enzyme solution, indicating that ABA Or 6-BA may be related to the promotion of the above enzyme synthesis. Combined with leaf water potential, stomatal resistance, chlorophyll content and photosynthetic rate, we can see that ABA or 6-BA can improve the photosynthesis of maize seedlings under water stress conditions, thereby improving their drought resistance, but the two modes of action are different .