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目的:分析miR-335在多种肿瘤组织与癌旁组织中的表达,预测其靶基因并进行相关生物信息学分析,为进一步研究miR-335在肿瘤中的调控机制提供理论基础。方法:分析miR-335的保守性及在多个肿瘤组织中的表达;预测miR-335靶基因,并使用DAIVID对miR-335靶基因进行生物信息学分析。结果:miR-335序列高度保守,在肝癌、肺癌、乳腺癌、肝内胆管癌、脂肪肉瘤中表达下调(P<0.05)。预测miR-335靶基因共34个,靶基因集合功能富集于细胞迁移、凋亡、转录调控,以及蛋白质分子连接、细胞骨架组成等生物学过程和分子功能(P<0.05);主要参与了轴突向导和黏着斑信号通路、黑素瘤疾病信号通路及TGF-β信号通路(P<0.05)。结论:miR-335在多种肿瘤中表达异常,且涉及多个生物学过程和信号转导通路,与肿瘤的发生发展密切相关。
OBJECTIVE: To analyze the expression of miR-335 in various tumor tissues and paracancerous tissues, to predict its target genes and to analyze related bioinformatics, so as to provide a theoretical basis for further study on the regulatory mechanism of miR-335 in tumors. Methods: The conservation of miR-335 and its expression in multiple tumor tissues were analyzed. The miR-335 target gene was predicted, and the bioinformatics analysis of miR-335 target gene was performed using DAIVID. Results: The miR-335 sequence was highly conserved and was down-regulated in HCC, lung cancer, breast cancer, intrahepatic cholangiocarcinoma and liposarcoma (P <0.05). A total of 34 miR-335 target genes were predicted, and the target gene pool was enriched in biological processes and molecular functions such as cell migration, apoptosis, transcriptional regulation, protein molecular attachment and cytoskeleton (P <0.05) Axon guidance and focal adhesion pathway, melanoma signaling pathway and TGF-β signaling pathway (P <0.05). Conclusion: miR-335 is abnormally expressed in many tumors and involves multiple biological processes and signal transduction pathways, which are closely related to the occurrence and development of tumors.