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目的 探讨雌激素在结肠癌细胞增殖调控中的作用及临床意义。方法 应用流式细胞术(FCM)检测 30例结肠癌、10例正常结肠组织雌激素受体 (ER)和细胞周期蛋白D1(CyclinD1)表达量。同时 ,用免疫组化法检测ER和增殖细胞核抗原 (PCNA)蛋白表达。结果 FCM定量检测表明 ,结肠癌组织ER表达量为 1.36± 0 .32 ,明显高于正常结肠组织 1.0 0± 0 .0 5 (P <0 .0 1) ;结肠癌组织CyclinD1表达量为 1.32± 0 .18,明显高于正常结肠组织 1.0 0± 0 .0 8(P <0 .0 1)。结肠癌组织ER表达量与CyclinD1表达量存在正相关关系 (r =0 .5 3 ,P <0 .0 1)。免疫组化染色表明 ,结肠癌组织ER表达阳性率为46 .7% (14/ 30例 ) ,明显高于正常结肠组织 (0 / 10例 ,P <0 .0 1) ;结肠癌组织PCNA蛋白表达阳性率为90 .0 % (2 7/ 30例 ) ,明显高于正常结肠组织 (0 / 10例 ,P <0 .0 1) ,且结肠癌组织ER表达等级与PCNA蛋白表达等级间存在正相关关系 (r=0 .43 ,P <0 .0 5 )。结论 雌激素在结肠癌发生、发展中有促进细胞增殖的作用 ,结肠癌很有可能是雌激素依赖性肿瘤。
Objective To investigate the role and clinical significance of estrogen in the regulation of colon cancer cell proliferation. Methods Flow cytometry (FCM) was used to detect the expression of estrogen receptor (ER) and cyclin D1 in 30 cases of colon cancer and 10 normal colon tissues. At the same time, ER and proliferating cell nuclear antigen (PCNA) protein expression was detected by immunohistochemistry. Results Quantitative detection of FCM showed that the expression level of ER in colon cancer tissues was 1.36±0.32, which was significantly higher than that in normal colon tissues (1.0 0±0.05) (P < 0.01). The expression level of CyclinD1 in colon cancer tissues was 1.32±. 0. 18, significantly higher than normal colon tissue 1.0 0 ± 0. 0 8 (P <0. 0 1). There was a positive correlation between the expression of ER and the expression of CyclinD1 in colon cancer tissues (r = 0.53, P < 0.01). Immunohistochemical staining showed that the positive rate of ER expression in colon cancer tissues was 46.7% (14/30 cases), which was significantly higher than that in normal colon tissues (0/10 cases, P < 0.01); PCNA protein in colon cancer tissues The positive rate of expression was 90.0 % (27/30 cases), which was significantly higher than that of normal colon tissues (0/10 cases, P < 0.01). There was a correlation between the expression level of ER and PCNA protein expression levels in colon cancer tissues. Positive correlation (r=0.43, P<0.05). Conclusion Estrogen can promote cell proliferation in the development and progression of colon cancer. Colon cancer is likely to be an estrogen-dependent tumor.