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从感染NIH3T3小鼠成纤维细胞上清液中分离小鼠腹水瘤病毒,用蔗糖密度梯度离心法纯化。从新鲜病毒中取小鼠腹水瘤病毒RNA,并用酚-氯仿抽提和蔗糖梯度分离。各组分的沉降系数(S)和分子量通过超速离心凝胶电泳分析测定。SRSV RNA主要由两组分组成,一是沉降系数约60～70S(热处理后约35S),还有少量18S和28S,另一是4～5S。35S组分在体外能指导无细胞蛋白的合成。
Mouse ascites virus was isolated from NIH3T3 mouse fibroblast supernatant and purified by sucrose density gradient centrifugation. Mouse ascite virus RNA was taken from fresh virus and separated by phenol-chloroform extraction and sucrose gradient. The sedimentation coefficient (S) and molecular weight of each component were determined by ultracentrifugation gel electrophoresis analysis. The SRSV RNA consists of two components, one with a sedimentation coefficient of about 60-70S (about 35S after heat treatment), a small amount of 18S and 28S, and the other of 4-5S. The 35S component can direct the synthesis of cell-free proteins in vitro.