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目的采用免疫组化PowerVision两步法检测137例大肠癌及其邻近正常黏膜组织中细胞周期素E(CyclinE)、抑制因子p27kipl和核抗原-67(Ki-67)。结果:大肠癌组织中CyclinE表达水平显著高于邻近正常黏膜组织,并与原发肿瘤浸润深度、区域淋巴结转移及TNM分期显著正相关(r分别为0.213、0.367、0.348,P均<0.05)。大肠癌组织中p27kipl表达水平显著低于邻近正常黏膜组织,并与原发肿瘤浸润深度、区域淋巴结转移及TNM分期显著负相关(r分别为-0.345、-0.269、-0.348,P均<0.01)。大肠癌组织中Ki-67表达水平显著高于邻近正常黏膜组织,并与区域淋巴结转移及TNM分期呈显著的正相关(r分别为0.218、0.172,P<0.05)。大肠癌组织中CyclinE与p27kipl表达水平显著负相关(r=-0.235,P<0.01);CyclinE和Ki-67表达水平显著正相关(r=0.243,P<0.01);大肠癌组织中p27kipl与Ki-67表达水平呈显著负相关(r=-0.172,P<0.01)。认为CyclinE和p27kipl是大肠癌发生及侵袭转移的正、负性因子,联合检测CyclinE、p27kip1和Ki-67有助于判断大肠癌的生物学行为及预后判断。
Objective To detect the expressions of CyclinE, p27kipl and Ki-67 in 137 cases of colorectal carcinoma and its adjacent normal mucosa by immunohistochemistry PowerVision two-step method. Results: The expression of CyclinE in colorectal cancer tissues was significantly higher than that in adjacent normal mucosa tissues. The expression of CyclinE was significantly correlated with the depth of invasion, regional lymph node metastasis and TNM stage (r = 0.213,0.367,0.348, P <0.05, respectively). The expression of p27kipl in colorectal cancer was significantly lower than that in adjacent normal mucosa tissues, and was negatively correlated with the depth of primary tumor invasion, regional lymph node metastasis and TNM stage (r = -0.345, -0.269, -0.348, P <0.01, respectively) . The expression of Ki-67 in colorectal cancer tissues was significantly higher than that in adjacent normal mucosa tissues, and was positively correlated with regional lymph node metastasis and TNM stage (r = 0.218,0.172, P <0.05). There was a significant negative correlation between the expression of CyclinE and p27kipl in colorectal carcinoma (r = -0.235, P <0.01), the positive expression of CyclinE and Ki-67 in colorectal carcinoma (r = 0.243, P <0.01) -67 expression was significantly negatively correlated (r = -0.172, P <0.01). It is considered that CyclinE and p27kipl are positive and negative factors of carcinogenesis and invasion and metastasis of colorectal cancer. Combined detection of CyclinE, p27kip1 and Ki-67 is helpful to judge the biological behavior and prognosis of colorectal cancer.