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生物体中普遍存在的14-3-3蛋白(也称GF14蛋白)能够参与植物的一系列应激响应过程。笔者以柽柳(Tamarix hispida)为材料,从6个柽柳cDNA文库中分离出5条GF14基因,依次命名为ThGF14a—ThGF14e。对5条GF14基因进行生物信息学研究,并利用qRT-PCR技术对胁迫处理后的基因表达模式进行分析。结果显示:ThGF14蛋白除了N和C末端的氨基酸外均含有一个高度保守的14-3-3结构域,这些ThGF14蛋白分属于ε-like和non-ε两种类型;大多数ThGF14基因在NaCl、PEG、4℃、CdCl_2处理不同时间(6,24,48和72 h)的叶和根中能够被诱导表达(表达量>2倍),且不同基因间对非生物胁迫应答表现出差异,其中ThGF14c基因在叶和根中不同胁迫条件下的表达水平均最高。研究表明,柽柳ThGF14基因能够参与植物的非生物胁迫应答响应过程。
The ubiquitous 14-3-3 protein (also called GF14 protein) in organisms is involved in a series of stress-responsive processes in plants. The author uses Tamarix hispida as material, and five GF14 genes are isolated from six tamarisk cDNA libraries, and named as ThGF14a-ThGF14e in turn. Five GF14 genes were subjected to bioinformatics analysis, and qRT-PCR was used to analyze the gene expression pattern after stress treatment. The results showed that: ThGF14 protein contains a highly conserved 14-3-3 domain except for the N and C-terminal amino acids, and these ThGF14 proteins belong to ε-like and non-ε types; most of the ThGF14 genes are expressed in NaCl, PEG, 4 and CdCl 2 treatments could induce expression in leaves and roots at different time points (6, 24, 48 and 72 h) (> 2 folds), and the differences in response to abiotic stresses between different genes were observed The expression level of ThGF14c gene under different stress conditions in leaves and roots was the highest. Studies have shown that tamarisk ThGF14 gene can participate in plant abiotic stress response process.