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以蚜虫差异抗性的大豆品种PI567598B(高抗)、绥农30(抗)、东农51(中抗)和Williams 82(感)为试验材料,分别于接种大豆蚜虫0,7,14和21 d时采集大豆叶片,利用q PCR对异黄酮合成关键酶基因苯丙氨酸解氨酶(PAL)、黄烷酮-3-3羟化酶(F3H)、大豆异黄酮合成基因1(ISF1)和大豆异黄酮合成基因2(ISF2)的表达量进行分析。结果表明:蚜虫取食前高抗品种PI567598B中PAL、F3H、ISF1和ISF2基因的相对表达量均高于感虫品种Williams 82;蚜虫取食后,抗蚜品种PI567598B和绥农30中PAL基因在蚜虫取食14 d时显著上调,在21 d时达到最大,F3H基因7 d时表达量最高,然后降低;IFS2表达量于蚜虫取食7 d后显著上调,在21 d时达到最大;中抗品种东农51中PAL、IFS2和F3H基因表达量在7 d时增加,但不显著;而感蚜品种Williams 82蚜虫取食后PAL和IFS2表达量增加但不显著,且相对表达量显著低于抗蚜品种,F3H基因表达量不增反降;抗感大豆品种蚜虫取食后IFS1基因表达均诱导不显著。研究表明,感蚜大豆品种蚜虫取食前后异黄酮合成关键酶基因表达量都比较低,而抗虫品种异黄酮合成关键酶基因表达量高,且防御响应持续时间长,符合其抗性差异。
Aphids differentially susceptible soybean varieties PI567598B (high resistance), Suinong 30 (resistant), Dongnong 51 (moderate resistance) and Williams 82 (flu) as test materials, were inoculated soybean aphids 0,7,14 and 21 The soybean leaves were collected at day d, and the key enzyme genes of phenylalanine ammonia lyase (PAL), flavanone -3-3 hydroxylase (F3H), isoflavone synthase 1 (ISF1) And soybean isoflavone synthesis gene 2 (ISF2) were analyzed. The results showed that the relative expression levels of PAL, F3H, ISF1 and ISF2 genes in PI567598B were higher than those in the susceptible strain Williams82. The aphids fed on the resistant varieties PI567598B and Suinong30 The aphids significantly increased on the 14th day and reached the maximum on the 21st day. The expression of F3H gene was highest on the 7th day and then decreased. The expression of IFS2 was significantly up-regulated on the 7th day after aphids’ feeding and reached the maximum on the 21st day. The expression levels of PAL, IFS2 and F3H in Dongnong 51 increased at 7 d, but not significantly. However, the relative expression levels of PAL and IFS2 in Williams 82 were significantly increased The anti-aphid varieties, F3H gene expression did not increase and decrease anti-susceptible soybean aphids fed IFS1 gene expression were not significant. The results showed that the expression levels of key enzyme genes of isoflavone biosynthesis were both low before and after aphid feeding on the aphid-susceptible soybean, while the expression of key enzyme genes related to isoflavone biosynthesis was high and the defense response lasted for a long time.