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目的探讨中性粒细胞弹性蛋白酶抑制剂(NEI)对急性百草枯中毒大鼠的治疗作用。方法 96只健康SPF级Wistar大鼠,随机分为对照组、模型组、治疗组,每组32只。以50mg/kg 20%百草枯水剂溶液一次性灌胃大鼠建立急性百草枯中毒动物模型,治疗组给予西维来司钠8mg/kg iv bid×3d。密切观察各组大鼠行为学改变,于染毒后第1、3、7、21天处死大鼠,采用ELISA法检测大鼠血清中血清白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)和中性粒细胞弹性蛋白酶(NE)浓度水平。结果与对照组相比,模型组各时间点血清IL-8、TNF-α、TGF-β1、NE水平较对照组均明显升高(P<0.05)。与模型组相比,治疗组在染毒后各时间点血清IL-8及TNF-α水平明显降低(P<0.05),血清TGF-β1水平第1、3天明显降低(P<0.05),血清NE第1、3、7天浓度水平较模型组明显下降(P<0.05)。结论 NEI对急性百草枯中毒大鼠有一定治疗作用,其机制可能与该药的抗炎作用有关。
Objective To investigate the therapeutic effect of neutrophil elastase inhibitor (NEI) on acute paraquat poisoning in rats. Methods 96 healthy SPF Wistar rats were randomly divided into control group, model group and treatment group, with 32 rats in each group. Acute paraquat poisoning animal model was established by intragastric administration of 50mg / kg 20% paraquat aqueous solution once a day, and the treatment group received svincalet sodium 8mg / kg iv bid × 3d. Closely observing the behavioral changes of rats in each group, rats were sacrificed on the 1st, 3rd, 7th and 21th day after exposure. The levels of serum interleukin-8 (IL-8), tumor necrosis factor -α (TNF-α), transforming growth factor-β1 (TGF-β1) and neutrophil elastase (NE) levels. Results Compared with the control group, the levels of IL-8, TNF-α, TGF-β1 and NE in the model group were significantly higher than those in the control group at each time point (P <0.05). Compared with the model group, the levels of serum IL-8 and TNF-α in the treatment group were significantly decreased (P <0.05) and the levels of TGF-β1 in the treatment group were significantly decreased on the 1st and 3rd days (P <0.05) The concentration of serum NE on the 1st, 3rd, 7th day was significantly lower than that of the model group (P <0.05). Conclusion NEI has a certain therapeutic effect on acute paraquat poisoning in rats, and its mechanism may be related to the anti-inflammatory effect of this drug.