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目的探讨佛波酯激活的蛋白激酶C与扭转蛋白A在亚细胞成分中的表达之间的关系。方法采用免疫荧光法观察扭转蛋白A在原代培养的神经元和小鼠胚胎成纤维细胞(NIH 3T3细胞)中的分布。运用蛋白质印迹法分析蛋白激酶C和扭转蛋白A在细亚细胞成分中的表达。结果扭转蛋白A在NIH 3T3细胞中的表达类似于神经元。扭转蛋白A在细胞质溶质、膜成分中均有分布。佛波酯活化蛋白激酶C后并不引起扭转蛋白A在细胞质成分和膜成分中表达含量的变化。结论扭转蛋白A可能是膜相关蛋白,细胞氧化应激中扭转蛋白A表达上调和重分布变化不是由佛波酯诱导的蛋白激酶C活化途径来实现的。鉴于扭转蛋白A表达上调具有潜在的治疗原发性早发扭转性肌张力障碍的前景,影响其分布和表达的分子机制需要进一步研究。
Objective To investigate the relationship between phorbol ester-activated protein kinase C and protein A in subcellular components. Methods The distribution of T helper protein A in primary cultured neurons and mouse embryonic fibroblasts (NIH 3T3 cells) was observed by immunofluorescence method. Western blotting was used to analyze the expression of protein kinase C and twistin A in subcellular components. Results The expression of T helper protein A in NIH 3T3 cells was similar to neurons. T helper protein A in the cytoplasmic solutes, membrane components are distributed. Phorbol ester activated protein kinase C does not cause changes in the expression of the protein content in the cytoplasm and membrane components of the twisted protein A changes. CONCLUSION: Troponin A may be a membrane-associated protein. The up-regulation and redistribution of T helper protein A in cellular oxidative stress are not mediated by phorbol ester-induced protein kinase C activation. In view of the up-regulated expression of Tropinin A has the potential to treat primary premature torsion dystonia, the molecular mechanisms that affect its distribution and expression need further study.