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目的初步探讨前列腺素E1(PGE1)对睾丸缺血再灌注后的功能保护作用及其可能的分子机制。方法建立大鼠睾丸缺血再灌注的病理模型(以扭转后复位为例)。随机分成A、B、C3组,每组12只。A、B、C组分别为对照、治疗和非治疗组。所有大鼠在手术45d后处死,取睾丸组织,肉眼观察其形态,称其重量(计算脏器系数),放入固定液以备行常规HE染色后镜下观察睾丸的病理改变,并在图像分析系统下测量精曲小管的直径,并对所有结果利用SPSS软件系统进行统计学处理。结果(1)肉眼观察形态:B组与A组相比差别不明显,B组与C组比较差别显著;(2)镜下观察形态:B组与A组相比差别不明显,B组与C组比较差别显著;(3)脏器系数比较:B组与A组差别无显著性(P>0.05)B组与C组差别显著(P<0.05);(4)精曲小管直径比较:B组与A组差别无显著性(P>0.05)B组与C组差别显著(P<0.05)。结论PGE1有减轻缺血再灌注导致的病理改变,可以运用于临床上治疗睾丸扭转复位后和睾丸移植术后,对睾丸功能恢复有明显促进作用。
Objective To investigate the functional protective effect of prostaglandin E1 (PGE1) on testis ischemia-reperfusion and its possible molecular mechanism. Methods The pathological model of testicular ischemia - reperfusion in rats was established. Randomly divided into A, B, C3 group, 12 in each group. Groups A, B and C were control, treatment and non-treatment groups respectively. All the rats were sacrificed 45 days after the operation, testicular tissue was taken and their morphology was observed with the naked eye. The weight of the testis was calculated (organ coefficient was calculated). After fixation, HE staining was used to observe the pathological changes of testis. The diameter of the seminiferous tubule was measured under the analysis system and all the results were statistically processed using the SPSS software system. Results (1) Macroscopic observation: There was no significant difference between group B and group A, and there was a significant difference between group B and group C. (2) Morphology under microscope: the difference between group B and group A was not obvious, There was significant difference between group C and group C (P <0.05); (4) The comparison of the diameter of seminiferous tubules: There was no significant difference between group B and group A (P> 0.05). The difference between group B and group C was significant (P <0.05). Conclusions PGE1 can reduce the pathological changes induced by ischemia-reperfusion, and can be used in the clinical treatment of testicular torsion and post-testicular transplantation, which can significantly promote the recovery of testicular function.