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目的:建立HPLC同时测定灯盏细辛注射液中绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸含量的方法。方法:Agilent Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相乙腈-0.1%甲酸水溶液,梯度洗脱,柱温30℃,检测波长327 nm,流速1.0 mL.min-1,进样量20μL。结果:绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、灯盏花乙素、3,5-二-O-咖啡酰奎宁酸、4,5-二-O-咖啡酰奎宁酸的线性范围分别为0.079～5.065(r=0.999 9),0.080～5.120(r=0.999 9),0.074～4.750(r=0.999 9),0.391～25.050(r=0.999 9),0.038～2.423(r=0.999 9),0.487～7.792 mg.L-1(r=0.999 8),加样回收率分别为96.72%(RSD 1.76%),96.64%(RSD 1.69%),101.81%(RSD 1.81%),98.16%(RSD 2.22%),99.02%(RSD 2.57%),97.02%(RSD 1.52%)。结论:方法简便、快捷,准确、重复性好,可为灯盏细辛注射液提供质量控制依据。
Objective: To establish an HPLC method for the simultaneous determination of chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, scutellarin, 3,5-di-O-caffeoylquinic acid , 4,5-di-O-caffeoylquinic acid content method. METHODS: The mobile phase consisted of acetonitrile-0.1% formic acid in water and gradient elution. The column temperature was 30 ℃. The detection wavelength was 327 nm and the flow rate was 1.0 mL · min-1. The mobile phase was Agilent Zorbax SB-C18 Injection volume 20μL. Results: Chlorogenic acid, caffeic acid, 1,3-O-dicaffeoylquinic acid, scutellarin, 3,5-di-O-caffeoylquinic acid, 4,5-di-O- The linear range of acyl quinic acid was 0.079 ~ 5.065 (r = 0.999 9), 0.080 ~ 5.120 (r = 0.999 9), 0.074 ~ 4.750 (r = 0.999 9), 0.391 ~ 25.050 (RSD 1.96%), 96.64% (RSD 1.69%) and 101.81% (RSD), respectively, with the average recoveries of ~ 2.423 (r = 0.999 9) and 0.487 ~ 7.792 mg.L- 1.81%), 98.16% (RSD 2.22%), 99.02% (RSD 2.57%), 97.02% (RSD 1.52%). Conclusion: The method is simple, rapid, accurate, reproducible, and can provide basis for quality control of Erigeron breviscapus injection.