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为筛选有利于提高渗透胁迫下小麦抗旱性的化学物质和最佳组合,以豫麦49为材料,在实验室中用15%PEG-6000模拟干旱,分别用不同浓度的磷酸二氢钾、赤霉素、葡萄糖、矮壮素及其混合液处理小麦种子。测定了各处理的芽长、根长、根数等形态指标和可溶性糖、丙二醛、脯氨酸含量等生理指标。结果表明,在PEG胁迫下,外源物质能不同程度地增加小麦幼苗的芽长、根长、根数等形态指标以及可溶性糖和脯氨酸含量,降低MDA的累积量,不同外源物质对水分亏缺的调节能力存在差异,其中外源物质的混合处理比单独处理的小麦表现出更强的抗旱能力。用0.2%KH2PO4、25mg/kg GA和500mg/kg葡萄糖的混合液处理小麦种子能增强小麦芽期的抗旱性。
In order to screen the best combinations of chemical substances that can improve the drought resistance of wheat under osmotic stress, Yumai 49 was used to simulate the drought in the laboratory with 15% PEG-6000, with different concentrations of potassium dihydrogen phosphate, Mice, glucose, chlormequat and their mixture of wheat seeds. The morphological indexes such as bud length, root length and root number, soluble sugar, malondialdehyde and proline content were measured. The results showed that under PEG stress, the exogenous substances could increase the shoot length, root length and root number of wheat seedlings, soluble sugar and proline content, reduce the accumulation of MDA, Water deficit regulatory capacity differences, in which the mixed treatment of exogenous substances than treated wheat showed more drought resistance. Treatment of wheat seeds with a mixture of 0.2% KH2PO4, 25 mg / kg GA and 500 mg / kg glucose increased wheat germination at drought-resistance.