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目的:观察小鼠小脑皮质片层化过程细胞迁移,探讨片层化、细胞迁移和reelin蛋白之间的关系。方法:不同年龄的小鼠172只,应用免疫荧光、Nissl和HE染色法对小脑形态结构及片层化、细胞迁移情况进行观察。结果:(1)小脑片层化:E15-E16的后脑主要由神经上皮构成,P0时小脑皮质基本形成了外颗粒层、分子层、浦肯野细胞层和颗粒层,P5时浦肯野细胞层形成2~3层细胞,分子层仍有大量细胞。P20时基本形成了小脑的典型三层结构。(2)细胞迁移过程:浦肯野细胞大约在E18时开始迁移,至P7时基本完成迁移,胞体形成均一的1~2层细胞结构。同时,小年龄时NeuN阳性细胞主要见于内颗粒层和外颗粒层,P7后阳性细胞只定位在内颗粒层,迁移基本停止。(3)野生鼠(WT)和reeler小鼠比较:约P14时,WT小鼠小脑形成分子层、浦肯野细胞层及颗粒层。但是在reeler小鼠,小脑分叶不良,浦肯野细胞未迁移至目的地,排列紊乱;内颗粒层(GL)细胞分布松散、不均一。结论:在小鼠小脑的片层化形成过程中,Reelin蛋白可能参与神经细胞增殖和迁移的调控过程。
OBJECTIVE: To observe the migration of mouse cerebellar cortex during laminarization and to explore the relationship between lamellarization, cell migration and reelin protein. Methods: A total of 172 mice of different ages were examined by immunofluorescence, Nissl and HE staining for morphological structure, lamellar and cell migration. Results: (1) Cerebellar laminarization: The posterior brain of E15-E16 was mainly composed of neuroepithelial epithelium. At the time of P0, the outer granular layer, molecular layer, Purkinje cell layer and granular layer were basically formed in the cerebellar cortex. At P5, Purkinje cells Layers formed 2 to 3 layers of cells, the molecular layer is still a large number of cells. P20 basically formed a typical three-tier structure of the cerebellum. (2) Cell migration process: Purkinje cells began to migrate around E18, migrated to P7, and cell bodies formed uniform cell layers 1 ~ 2. At the same time, NeuN-positive cells were mainly found in the inner granular layer and the outer granular layer in younger age. The positive cells in P7 were only located in the inner granular layer, migration basically stopped. (3) Comparison of WT and reeler mice: At about P14, WT mouse cerebellum formed molecular layer, Purkinje cell layer and granular layer. However, in reeler mice, the cerebellar lobes were poor, Purkinje cells did not migrate to their destination, and disorganized; the inner granulosa layer (GL) cells were loosely and heterogeneously distributed. Conclusion: Reelin may be involved in the regulation of neural cell proliferation and migration in the process of cerebellar laminarization.