论文部分内容阅读
桃砧木离体无性繁殖技术为快速繁殖新无性系提供了一种新手段。这种技术已应用于苹果砧木的繁殖。Skirvin等离体繁殖了桃嫩枝,但仅少数植株可生根。在意大利,一些李属果品己用离体培养技术进行商业生产。Negueroles等报道了用离体技术生产了GF305桃砧木。本试验是研究生产“Nemag-uard”桃砧木的离体技术。于5月下旬选取生长活跃的“Nemaguard”嫩枝(约15cm)。在实验室中切下包括顶端分生组织和1～2个叶原基的茎尖(约5mm),浸在加有0.1％吐温20的10％Clorox(0.5％次氯酸钠)中10分钟,用除去离子的无菌蒸馏水(SDD)冲洗2次,每次5分钟,
Asexual propagation of peach rootstocks in vitro provides a new method for rapid propagation of new clones. This technique has been applied to the propagation of apple rootstocks. Skirvin breeds nectarines in vitro, but only a few plants can root. In Italy, some Prunus fruits have been commercialized using in vitro culture techniques. Negueroles et al. Reported the production of GF305 peach rootstock using ex vivo techniques. This is an in vitro study to study the production of “Nemag-uard” peach rootstocks. Select the active “Nemaguard” shoots (about 15cm) in late May. Shoot tips (about 5 mm) including apical meristem and 1-2 leaf primordia were excised in the laboratory, immersed in 10% Clorox (0.5% sodium hypochlorite) plus 0.1% Tween 20 for 10 minutes, Of sterile distilled water (SDD) rinse 2 times, each 5 minutes,