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[目的]了解2010年春季成都地区婴幼儿腹泻中杯状病毒感染率、基因型和优势毒株。[方法]收集四川大学华西第二医院2010年春季(3～5月)腹泻婴幼儿的粪便标本,采用逆转录-聚合酶链反应(RT-PCR)方法检测,阳性扩增片段通过T-A克隆测序,最后进行核苷酸序列的进化分析。[结果]诺如病毒NoV的检出率为12%(9/75),包括GⅠ组2例,GⅡ组7例,未检出札如病毒SaV。其中7株GⅡ组与标准毒株Lordsale相似性为93.0%～94.7%,最为接近,结合进化分析显示皆为GII-4基因型。[结论]杯状病毒是导致成都地区婴幼儿腹泻的重要病原之一,其主要流行型为NoVGⅡ-4型。
[Objective] To understand the prevalence of calicivirus, genotype and predominant strains of infantile diarrhea in Chengdu in the spring of 2010. [Methods] The stool samples of infants and young children with diarrhea in spring 2010 (March-May) in West China Second Hospital of Sichuan University were collected and detected by RT-PCR. The positive amplified fragments were sequenced by TA cloning Finally, the nucleotide sequence of the evolutionary analysis. [Results] The detection rate of Novo virus NoV was 12% (9/75), including 2 cases in GⅠ group and 7 cases in GⅡ group. Sapporo virus SaV was not detected. Among them, the similarity of the 7 strains of GⅡ to the standard strain Lordsale was 93.0% ~ 94.7%, which was the closest, and the evolutionary analysis showed that all of them were GII-4 genotypes. [Conclusion] Calicivirus is one of the most important pathogens causing infantile diarrhea in Chengdu. Its main epidemic type is NoVGⅡ-4.