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目的:探讨胃癌组织中浸润巨噬细胞与微血管密度的相关性及其在胃癌侵袭转移中的作用。方法:应用免疫组化SP法,用抗CD68 和抗CD34单克隆抗体对102例胃癌组织进行标记分析,并应用计算机辅助图像分析系统对血管新生的面积进行定量分析。结果:巨噬细胞浸润与胃癌分化程度、浸润深度、淋巴结转移和TNM分期有关,且有显著性差异(p<0.01,p<0.05,p<0.05, p<0.05)。MVD与分化程度、浸润深度、淋巴结转移,TNM分期相关,且有显著性差异(p<0.01)。巨噬细胞计数与MVD呈正相关(r=0.961,p<0.01)。结论:联合检测巨噬细胞浸润度及MVD定量分析可提供胃癌恶性转归更有价值的指标。
Objective: To investigate the correlation between infiltrating macrophages and microvessel density in gastric cancer and its role in the invasion and metastasis of gastric cancer. Methods: 102 cases of gastric cancer were labeled by anti-CD68 and anti-CD34 monoclonal antibodies by immunohistochemical SP method. The area of angiogenesis was quantitatively analyzed by computer-assisted image analysis system. Results: The infiltration of macrophages was related to the degree of differentiation, depth of invasion, lymph node metastasis and TNM stage (P <0.01, p <0.05, p <0.05, p <0.05). MVD was correlated with the degree of differentiation, depth of invasion, lymph node metastasis and TNM stage (p <0.01). Macrophage counts were positively correlated with MVD (r = 0.961, p <0.01). Conclusion: The combined detection of macrophage infiltration and MVD quantitative analysis can provide a more valuable indicator of malignancy of gastric cancer.