论文部分内容阅读
以狂犬病街毒株为攻击病毒建立检测狂犬病单克隆抗体中和活性的方法。小鼠脑内接种分离扩增狂犬病阳性标本24株;分离到的街毒株在N2A细胞进行培养及病毒滴度测定,选择能够适应N2A细胞生长且滴度较高的街毒株建立病毒库;建立方法后对TRN006单克隆抗体进行3次检测,评价其重复性。24份狂犬病阳性标本均分离成功;只有15株病毒能够适应N2A细胞,并在细胞上形成荧光灶;最终选取10株滴度较高病毒建立病毒库,该病毒库覆盖了中国9个不同省份及四个中国毒株群;用其中三种病毒对狂犬病单克隆抗体进行中和活性检测3次,T检验发现其具有良好的重复性。
A rabies street virus strain was used as the target virus to establish a method for detecting the neutralizing activity of rabies monoclonal antibody. Twenty-four rabies positive rabies positive strains were inoculated intracerebrally in mice, and the isolated strains were cultured in N2A cells and the virus titer was determined. The virus strains were selected to be street strains capable of adapting to N2A cell growth and high titers. After establishing the method, TRN006 monoclonal antibody was tested 3 times to evaluate its repeatability. Twenty-four rabies positive samples were successfully isolated. Only 15 viruses could adapt to N2A cells and form fluorescence foci on the cells. Finally, 10 strains with higher titer were selected to establish a virus database covering 9 different provinces in China and Four Chinese strain groups. The neutralizing activity of rabies monoclonal antibody was tested three times with three kinds of viruses, and the T test showed that it has good repeatability.