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Exactly capturing three dimensional (3D) motion information of an object is an essential and important task in computer vision, and is also one of the most difficult problems. In this paper, a binocular vision system and a method for determining 3D motion parameters of an object from binocular sequence images are introduced. The main steps include camera calibration, the matching of motion and stereo images, 3D feature point correspondences and resolving the motion parameters. Finally, the experimental results of acquiring the motion parameters of the objects with uniform velocity and acceleration in the straight line based on the real binocular sequence images by the mentioned method are presented.