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血小板计数对血液系统疾病的诊断和疗效观察均具有重要意义。但试剂、操作技术及计数误差均可影响血小板的计数结果。我们试用直接计数及涂片观察相结合的方法,取得较好的效果。临床资料:选用我院1984年12月至1991年10月进行骨髓移植的17例患者,对每一例患者检查的次数从移植的第1天一直到血小板恢复正常为止。试剂与方法:(1)分别取0.85％生理盐水和/或1％草酸铵溶液各2ml,采血方法及检查步骤均按传统法。(2)血片经瑞氏染色后观察,用油镜观察涂片中100个视野的血小板平均数量。同一病人每天同
Platelet count blood disease diagnosis and efficacy are of great significance. However, reagents, operating techniques and counting errors can affect platelet count results. We try to direct counting and smear combined with the method of observation, and achieved good results. Clinical data: 17 patients who underwent bone marrow transplantation in our hospital from December 1984 to October 1991 were selected, and the number of examination for each patient from the first day of transplantation until the platelet returned to normal. Reagents and methods: (1) were taken 0.85% saline and / or 1% ammonium oxalate solution of 2ml, blood collection methods and inspection procedures are based on traditional methods. (2) Blood samples were observed after Wright’s staining, and the average number of platelets in 100 fields in the smear was observed with an oil microscope. The same patient every day