论文部分内容阅读
目的探讨双歧杆菌对新生大鼠坏死性小肠结肠炎(NEC)模型的保护作用及分子机制。方法将45只新生大鼠随机分为对照组(n=14)、NEC组(n=16)和干预组(n=15)。对照组由哺乳期母鼠代乳,NEC组和干预组给予连续3天人工喂养和每天2次缺氧+寒冷刺激,干预组额外给予每天2次青春型双歧杆菌(每次1×108CFU)。比较3组肠道病理评分、NEC发生率,采用荧光定量PCR方法检测Toll样受体4(TLR4)、Toll作用蛋白(TOLLIP)及单Ig区IL-1相关受体(SIGIRR)mRNA表达并进行组间比较。结果对照组、NEC组和干预组肠道病理评分分别为0分、(2.4±1.0)分和(1.3±0.6)分;NEC发生率分别为0(0/14)、77.8%(7/9)和23.1%(3/13);TLR4 mRNA相对表达量分别为1.00±0.38、3.64±1.73和1.78±0.70;TOLLIP mRNA相对表达量分别为1.00±0.85、0.23±0.15和0.86±0.62;SIGIRR mRNA相对表达量分别为1.00±0.58、0.27±0.12和0.71±0.49。干预组与NEC组肠道病理评分和NEC发生率比较差异均有统计学意义(P<0.05)。NEC组TLR4 mRNA表达高于对照组和干预组,TOLLIP mRNA、SIGIRR mRNA表达低于对照组和干预组(P<0.05);干预组与对照组TLR4、TOLLIP和SIGIRR mRNA表达差异均无统计学意义(P>0.05)。结论双歧杆菌对新生大鼠NEC模型肠道起保护作用,可能通过下调肠道组织TLR4 mRNA表达及上调TOLLIP和SIGIRR mRNA表达发挥作用。
Objective To investigate the protective effect of Bifidobacterium on neonatal rat model of necrotizing enterocolitis (NEC) and its molecular mechanism. Methods 45 neonatal rats were randomly divided into control group (n = 14), NEC group (n = 16) and intervention group (n = 15). In the control group, breastfeeding maternal mice were given milk, NEC group and intervention group were given 3 consecutive days of artificial feeding and 2 times of hypoxia + cold stimulation every day. In the intervention group, Bifidobacterium adolescentis (1 × 108 CFU) . The intestinal histopathological score and the incidence of NEC were compared between the three groups. The mRNA expression of Toll-like receptor 4 (Toll-like receptor 4), Toll-stimulating protein (TOLLIP) and IL-1 related receptor (SIGIRR) Comparison between groups. Results The scores of intestinal pathology in control group, NEC group and intervention group were 0, 2.4 ± 1.0 and 1.3 ± 0.6 respectively. The incidence of NEC was 0 (0/14) and 77.8% (7/9) respectively ) And 23.1% (3/13) respectively. The relative expression of TLR4 mRNA was 1.00 ± 0.38, 3.64 ± 1.73 and 1.78 ± 0.70, respectively. The relative expression of TOLLIP mRNA was 1.00 ± 0.85, 0.23 ± 0.15 and 0.86 ± 0.62, respectively The relative expression levels were 1.00 ± 0.58, 0.27 ± 0.12 and 0.71 ± 0.49, respectively. The difference of intestinal pathological score and NEC between intervention group and NEC group was statistically significant (P <0.05). The expression of TLR4 mRNA in NEC group was higher than that in control group and intervention group, while the expressions of TOLLIP mRNA and SIGIRR mRNA were lower than those in control group and intervention group (P <0.05). There was no significant difference in TLR4, TOLLIP and SIGIRR mRNA expression between NEC group and control group (P> 0.05). Conclusion Bifidobacterium plays a protective role in the intestinal tract of neonatal rat model of NEC, which may play a role in down-regulation of TLR4 mRNA expression and up-regulation of TOLLIP and SIGIRR mRNA expression in intestinal tissue.