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目的探讨IL-2/Fc融合表达后对HBV preS2S基因疫苗诱导免疫反应的佐剂效应。方法采用HBV preS2S DNA疫苗作为基础免疫,重组质粒pcDNA3．1IL-2/Fc作为佐剂加强免疫BALB/c小鼠．采用0、2、4周的方案接种,检测各次接种后抗体水平。初次免疫后7周,测定免疫脾细胞的杀伤活性、增殖活性和细胞因子的分泌水平。结果pcDNA3．1IL-2/Fc作为佐剂在HBV preS2S注射3d后免疫组小鼠抗体滴度、免疫脾细胞的杀伤活性和增殖活性、T_H1型细胞因子的分泌水平,均比各对照组明显增强。结论IL-2/Fc是有效的HBV preS2S DNA疫苗佐剂之一。
Objective To explore the adjuvant effect of IL-2 / Fc fusion on HBV-induced immune response induced by preS2S gene vaccine. Methods HBV preS2S DNA vaccine was used as the basic immunogen and BALB / c mice were immunized with the recombinant plasmid pcDNA3.1 IL-2 / Fc as an adjuvant. 0,2,4 weeks of the program inoculation, testing each antibody level after inoculation. Seven weeks after the first immunization, the killing activity, proliferative activity and cytokine secretion of the immunized spleen cells were measured. Results The antibody titers, cytotoxicity and proliferative activity of splenocytes in mice immunized with pcDNA3.1 IL-2 / Fc as adjuvant were significantly higher than those in control group . Conclusion IL-2 / Fc is one of the effective HBV preS2S DNA vaccine adjuvants.