论文部分内容阅读
抗微丝蚴药物对犬恶丝虫化疗试验,过去惯用实验感染的动物或自然感染的犬类,但是建立开放性实验感染的缺点需要的时间长、花费大,并且缺乏自然感染犬的遗传、营养、生理、寄生虫学和免疫状况的资料。因此,作者认为建立开放性实验感染的时间短和适应环境的模型系统,将有助于加速抗微丝蚴化合物的筛选。作者在无菌条件下,从自然感染犬恶丝虫的犬心和肺动脉中取得犬恶丝虫成虫,置pH7.6的含有HEPES(3.95g/L)、重碳酸钠(2g/L)及庆大霉素(50mg/ml)的RPMI-1640培养液洗涤后,移入新的培养容器,经两个半月即可获得大量的微投蚴。应用这些微
Anti-microfilarial drugs are resistant to dirnecidae chemotherapy, past-used animals or naturally-infected dogs, but the drawbacks of establishing an open experimental infection can be time consuming, costly, and lacking the genetic predisposition to naturally infected dogs, Nutrition, Physiology, Parasitology and Immune Status Information. Therefore, the authors believe that the establishment of a model system for a short period of time and adaptation to the environment of an open experimental infection will help to speed up the screening of anti-microfilaria compounds. Under aseptic conditions, the adult D. caninum was obtained from canine heart and pulmonary arteries that naturally infected D. hellensis. HEPES (3.95 g / L), sodium bicarbonate (2 g / L) at pH 7.6 and After the gentamicin (50mg / ml) RPMI-1640 medium was washed, it was transferred to a new culture container and a large number of micro-pollination larvae were obtained after two and a half months. Apply these micro