Effect of Sini Decoction plus Ginseng on COVID-19 based on network pharmacological analysis

• 作 者: Xin-Hua Zhao Dong-Xu Zhang Tian-Jing Zhao Xue-Ying Pang Song-Jiang Liu
• 机 构: Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,China;The Eighth Medical Cente
• 出 处: 海南医科大学学报（英文版）
• 发表日期: 2021年19期
• 关键词: COVID-19 Network pharmacology Sini Decoction plus Ginseng Mechanism of action

Objective: To explore the effect of Sini Decoction plus Ginseng on COVID-19 based on network pharmacological analysis. Methods: TCMSP platform was used to search the compounds of Sini Decoction plus Ginseng with oral bioavailability(OB)≥30％ and drug-likeness(DL)≥0.18,and the results were input into the UniProt database and converted into standard target names;Genecards and OMIM database were used to find COVID-19 target, and the intersection targets were obtained and Venn diagram was drawn. Cytoscape 3.7.2 was applied to make the network diagram of composition-disease-target;The interaction database platform-String was used to analyze the target protein interaction, and the data were input into the software of Cytoscape 3.7.2 to make the network diagram;David database was used to analyze the accumulation of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathways of drug-disease intersection targets. The component target pathway network was constructed by using Cytoscape 3.7.2. Results: Altogether, 112 active components of Sini Decoction plus Ginseng were screened, corresponding to 234 targets. Besides, 261 COVID-19 targets were obtained after screening, and the Venn map showed that 50 targets were intersected. Quercetin, kaempferol,naringenin,and β-sitosterol were found to have higher moderate values shown by the component-disease-target network. The target proteins with high PPI network analysis value were IL-6,MAPK8,MAPK3, MAPK1,TP53,TNF,and CASP3. GO enrichment function analysis showed that 341 biological processes, 33 cell components,and 50 molecular functions were involved,which showed inflammatory reaction,cell response to lipopolysaccharide,exogenous apoptosis signal pathway,positive regulation of RNA polymerase Ⅱ promoter transcription,cytokine activity,chemokine activity,heme binding,and other biological processes. KEGG signaling pathway enrichment function analysisshowed that there were 112 signaling pathways. It included TNF signaling pathway,tuberculosis,influenza A,PI3K Akt signaling pathway,HIF-1 signaling pathway,and Toll-like receptor signaling pathway. According to the component-target-pathway network diagram,quercetin,kaempferol,naringenin, and β -sitosterol in Sini Decoction plus Ginseng may act on IL-6,MAPK8, MAPK3, MAPK1,TP53,TNF,CASP3,and other targets through TNF signaling pathway,MAPK signaling pathway,Tolllike receptor signaling pathway,PI3K-Akt signaling pathway,and HIF-1 signaling pathway. Conclusion:Sini Decoction plus Ginseng can affect COVID-19 through multiple active components,multiple targets,and multiple pathways.