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目的:通过考察糖槭叶多糖(ASP-A)和糖槭果多糖(ASP-B)对脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7分泌细胞因子的影响,明确其抗炎作用机制。方法:MTT法测定不同浓度两类多糖对RAW264.7细胞的毒性作用,酶联免疫吸附试验(ELISA)检测细胞上清液中一氧化氮(NO)、肿瘤坏死因子-1α(TNF-1α)、前列腺素E2(PGE2)、白细胞介素-1β(IL-1β)的含量。结果:糖槭多糖能够抑制LPS诱导的RAW264.7细胞释放细胞炎症因子NO,TNF-1α,PGE2,IL-1β的分泌量。结论:ASP-A和ASP-B在浓度小于200 mg·L-1对RAW264.7细胞无显著毒性,且可以通过抑制细胞因子NO,TNF-1α,PGE-2,IL-1β的分泌发挥抗炎作用。
OBJECTIVE: To investigate the effect of ASP-A and ASP-B on cytokines secreted by lipopolysaccharide (LPS) -induced RAW264.7 in murine macrophages and its anti-inflammatory effects mechanism. Methods: The toxic effects of two polysaccharides with different concentrations on RAW264.7 cells were determined by MTT assay. The levels of nitric oxide (NO), tumor necrosis factor-1α (TNF-α) , Prostaglandin E2 (PGE2), interleukin-1β (IL-1β) content. Results: Polysaccharides from Maple Leaf could inhibit the secretion of NO, TNF-1α, PGE2 and IL-1β in LPS-induced RAW264.7 cells. CONCLUSION: ASP-A and ASP-B showed no significant cytotoxicity on RAW264.7 cells at a concentration of less than 200 mg · L-1 and could exert anti-tumor activity by inhibiting the secretion of cytokines NO, TNF-α, PGE-2 and IL- Inflammation.