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目的探讨女性解脲脲原体(Ureaplasma urealyticum,Uu)感染与其男性伴侣Uu感染的关系,为临床诊治Uu感染提供依据。方法对2010年2月至2011年3月就诊并排除其他病原体感染的有症状且Uu阳性女性334例,无症状但Uu阳性女性61例和同期男性伴侣395例,分别取宫颈管或阴道、尿道分泌物进行Uu实时荧光定量-聚合酶链反应(real-time fluorescence quantitative-Polymerase Chain Reaction,FQ-PCR)基因检测和PCR反向斑点杂交(PCR-RDB)基因分群检测。结果 395例男性伴侣中,203例(51.39%)经FQ-PCR检测为Uu阳性,其中,有症状和无症状女性的男性伴侣Uu阳性率分别为56.3%(188/334)和24.6%(15/61),差异有统计学意义(χ2=19.50,P<0.01)。基因分群显示,有症状女性感染者生物1群、生物2群和混合群各占一定比例,但以生物1群占优势,而无症状女性感染者绝大部分以生物1群为主,占86.9%(53/61);有症状女性的男性伴侣中,有尿道炎症状者绝大部分(98.7%,147/149)为生物2群和混合群感染,无尿道炎症状者则绝大部分(94.9%,37/39)为生物1群感染;而无症状女性的男性伴侣均无尿道炎症状,感染主要以生物1群为主,占80.0%(12/15)。进一步分析发现,女性感染生物1群时其男性伴侣发生感染的比例较低,而女性感染生物2群和混合群时,其男性伴侣感染同类基因群的比例则高达98.6%(71/72)和73.0%(84/115)。结论无论女性感染生物1群还是生物2群,其男性伴侣感染的主要是生物2群。因此,在女性Uu感染者及其男性伴侣中进行Uu基因分群分析,不但可指导临床制定有针对性的治疗方案,而且对监测Uu的基因变化、追踪传染源、分析流行趋势有重要意义。
Objective To investigate the relationship between female Ure infection and its female partner Uu infection and to provide evidence for the clinical diagnosis and treatment of Uu infection. Methods From February 2010 to March 2011, 334 women with symptomatic and Uu-positive symptoms, 61 asymptomatic Uu-positive women and 395 male partners at the same period were enrolled in the study and excluded from other pathogens. Cervical or vaginal, urethral The secretions were detected by Uu real-time fluorescence quantitative-polymerase chain reaction (FQ-PCR) and PCR-reverse dot blot (PCR-RDB). Results Of the 395 male partners, 203 (51.39%) were positive for Uu by FQ-PCR. The positive rates of Uu were 56.3% (188/334) and 24.6% (15%) in symptomatic and asymptomatic women / 61), the difference was statistically significant (χ2 = 19.50, P <0.01). Gene grouping showed that the number of biological group 1, biological group 2 and mixed group accounted for a certain proportion of women infected with symptomatic disease, but dominated by biological group 1, whereas the majority of those with asymptomatic female infection were mainly group 1, accounting for 86.9 % (53/61). Among the male partners with symptomatic women, the majority of those with urethritis symptoms (98.7%, 147/149) were infected with two groups of organisms and the mixed group. Most of the patients without urethritis symptoms 94.9% and 37/39), respectively. However, asymptomatic female males had no symptoms of urethritis. The majority of infections were mainly organism group, accounting for 80.0% (12/15). Further analysis showed that the infection rate of male partners was lower in women infected with one group of organisms, while the percentage of female partners infected with the same gene group was 98.6% (71/72) in women infected with two and mixed groups 73.0% (84/115). Conclusion Whether women infected with one or two biological groups, their male partners are mainly infected with two groups of organisms. Therefore, the Uu gene cluster analysis in female Uu infected persons and their male partners not only can guide the clinical development of targeted treatment programs, but also has important significance in monitoring the genetic changes of Uu, tracing the source of infection and analyzing epidemic trends.