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目的比较维持血液透析患者和正常健康人血清中肿瘤坏死因子α(TNF-α)浓度的差异,探讨TNF-α在维持血液透析患者的意义。方法采用酶联免疫吸附法测定40名维持血液透析患者和40名正常健康成人血清中TNF-α的浓度,同时测定所有研究对象的血清尿素氮(BUN)、肌酐(CREA)、尿酸(UA)、超敏C反应蛋白(hs-CRP)及血清胱氨酸蛋白酶抑制剂C(Cyst C),并用统计学方法对其数据进行分析。结果维持血液透析患者血清TNF-α水平(23.02±0.22)pg/ml明显高于健康对照组(10.16±1.19)pg/ml,差异有统计学意义(P<0.01)。CRP、BUN、CREA、UA和Cyst C也较健康对照组显著升高(P<0.01)。相关性分析结果显示维持血液透析患者血清中TNF-α与BUN(r=0.43,P<0.05),CREA(r=0.79,P<0.01)呈正相关且差异有统计学意义,但与CRP(r=0.13)、Cyst C(r=0.26)、UA(r=0.01)无相关性。结论 TNF-α是维持血液透析患者的一个重要炎症介质,且联合BUN和CRE的测定可以监测其病情。
Objective To compare the maintenance of serum TNF-α levels in hemodialysis patients and normal healthy people, and to explore the significance of TNF-α in maintaining hemodialysis patients. Methods Serum levels of TNF-α in 40 hemodialysis patients and 40 normal healthy adults were measured by enzyme-linked immunosorbent assay. Serum urea nitrogen (BUN), creatinine (CREA), uric acid (UA) , High-sensitivity C-reactive protein (hs-CRP) and serum cystatin C (Cyst C), and their data were analyzed by statistical methods. Results The level of TNF-α in maintenance hemodialysis patients (23.02 ± 0.22) pg / ml was significantly higher than that in healthy controls (10.16 ± 1.19) pg / ml, the difference was statistically significant (P <0.01). CRP, BUN, CREA, UA and Cyst C were significantly higher than those in healthy controls (P <0.01). Correlation analysis showed that there was a positive correlation between serum TNF-α and BUN (r = 0.43, P <0.05) and CREA (r = 0.79, = 0.13), Cyst C (r = 0.26), UA (r = 0.01). Conclusion TNF-α is an important inflammatory mediator in the maintenance of hemodialysis patients, and the combination of BUN and CRE can monitor the disease.