论文部分内容阅读
实验用免疫细胞化学技术观察了大鼠鞘内分别注入蛋白激酶(PKC)抑制剂Chelerythrine(Chel)、纳洛酮(Nal)、或二者同时注入后,由后脚掌注射福尔马林引起的脊髓腰膨大背角中c-fos蛋白样免疫活性(Fos-LI)神经元数目的改变。结果发现:(1)鞘内注入Chel可显著降低福尔马林注射侧脊髓背角中Fos-LI神经元的数目,同空白对照组(鞘内注入生理盐水或10％的DMSO)相比,降低60.3％(P<0.001):(2)鞘内注入Nal后,福尔马林注射侧背角中Fos-LI神经元显著增加,同对照组相比,增加46.0％(P<0.01),而以背角深层增加最为明显;(3)在鞘内同时注入Chel和Nal后,与单独注入Nal组相比,脊髓背角中Fos-LI神经元的数目显著降低(降低53.2％),此数值与上述单独注入Chel时引起Fos-LI神经元降低的百分率近似。结果提示:(1)PKC只参与脊髓背角中部分Fos-LI神经元中c-fos蛋白的表达;(2)PKC可能不参与背角中同时激活的μ-(以及部分δ-)阿片受体对脊髓伤害性感受的调制。
Experimental immunocytochemistry was used to observe the effects of injecting formalin after injection of Chelerythrine (Chel), Naloxone (Nal), a protein kinase (PKC) inhibitor, Changes of c-fos protein-like immunoreactive (Fos-LI) neurons in spinal dorsal horn. The results showed that: (1) Intrathecal injection of Chel significantly reduced the number of Fos-LI neurons in the spinal dorsal horn on the injection side of formalin. Compared with the blank control group (intrathecal saline or 10% DMSO) (P <0.001). (2) Fos-LI neurons in the dorsal horn of formalin injection increased significantly after intrathecal injection of Nal compared with the control group (46.0%, P <0.01) (3) In the intrathecal injection of Chel and Nal, the number of Fos-LI neurons in spinal dorsal horn decreased significantly (by 53.2%) compared with that of Nal alone Values were similar to the percentages of Fos-LI neurons that caused a decrease in these Chel alone injections. The results suggest that: (1) PKC only participates in the expression of c-fos protein in some Fos-LI neurons in the dorsal horn of spinal cord; (2) PKC may not participate in the activation of μ- (and some δ-) opioids in the dorsal horn Modulation of nociception of the spinal cord.