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为阐明IL-1对粒系细胞和红系细胞的作用,我们通过对小鼠骨髓及外周血的研究探讨了IL-1对粒系和红系造血细胞的调节作用.结果表明:IL-1单剂量腹腔注射后第7天红系造血细胞明显减少,外周血网织红细胞在第8天显著下降.在5～10万U/kg剂量范围内IL-1明显促进粒系细胞的增殖.应用流式细胞仪对DNA分析显示IL-1并不引起全骨髓细胞DNA的变化,但大体积细胞在注射IL-1后第3天S期细胞明显增多.我们的结果表明IL-1抑制红系造血细胞的分化增殖,在适当的剂量范围内促进粒系细胞的增殖和分化成熟.其作用的分子基础是诱导造血细胞的细胞周期变化.
To clarify the effect of IL-1 on myeloid cells and erythroid cells, we investigated the regulatory effect of IL-1 on myeloid and erythroid hematopoietic cells by studying bone marrow and peripheral blood in mice.The results showed that IL-1 On the 7th day after single-dose intraperitoneal injection, hematopoietic cells in erythrocytes were significantly reduced, and the number of peripheral blood reticulocytes significantly decreased on day 8. IL-1 significantly promoted the proliferation of granulosa cells in the dose range of 500 ~ 100000 U / kg. Flow cytometry analysis of DNA showed that IL-1 did not cause DNA changes in whole myeloid cells, but large cells showed a marked increase in S phase on day 3 after injection of IL-1.Our results show that IL-1 inhibits erythroid Differentiation and proliferation of hematopoietic cells promote the proliferation and differentiation and maturation of granulosa cells in the proper dose range.The molecular basis of their action is to induce cell cycle changes of hematopoietic cells.