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为观察酒精性肝损伤后大鼠肝内酶组织化学变化，以５６°白酒给大鼠灌胃，５ｄ后取肝组织做酶组织化学染色，与对照组比较，显示除肝内出现脂变、糖原减少外，醛脱氢酶、琥珀酸脱氢酶、乳酸脱氢酶、谷氨酸脱氢酶及三磷酸腺苷酶活性呈不同程度减弱；乙醇脱氢酶、酸性磷酸酶和葡萄糖６磷酸酶活性增强，提示线粒体酶及胞浆质酶是酒精性肝损伤中早期具特征性的标志酶。这种变化差异的意义在于上述酶的变化反映了肝细胞内乙醇代谢主要通路的改变，同时也可表明高毒性代谢产物乙醛产生的水平，对于判断肝细胞损伤的程度具有重要作用。
In order to observe the intrahepatic enzymatic histochemical changes in rats with alcoholic liver injury, the rats were inoculated intragastrically with 56 ° liquor and the liver tissues were harvested for enzyme histochemical staining 5 days later. Compared with the control group, Aldehyde dehydrogenase, succinate dehydrogenase, lactate dehydrogenase, glutamate dehydrogenase and adenosine triphosphate activity were weakened to varying degrees; alcohol dehydrogenase, acid phosphatase and glucose 6 phosphate Enzyme activity increased, suggesting that mitochondrial enzymes and cytosolic enzymes in alcoholic liver injury in early signs of a characteristic enzyme. The significance of this difference in variation lies in that the changes of the above enzymes reflect the change of the main pathway of ethanol metabolism in hepatocytes and also indicate that the level of acetaldehyde produced by the highly toxic metabolite plays an important role in judging the degree of hepatocyte damage.