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目的建立基于悬液芯片的乙肝病毒(HBV)基因型检测方法。方法根据现有HBV 8个基因型的DNA序列信息,设计并合成相关引物及探针,首先进行PCR扩增,产物与核酸探针微球组杂交后检测荧光信号值。结果建立的悬浮芯片方法检测HBV-A、HBV-E、HBV-G的敏感性为9DNA拷贝,HBV-B、HBV-C、HBV-D、HBV-F、HBV-H的敏感性为90DNA拷贝。检测结果与荧光PCR方法相比,差异无统计学意义。结论建立了可同时检测8个HBV基因型的悬液芯片检测方法,为快速筛查和鉴定HBV提供了新的手段。
Objective To establish a method for detecting hepatitis B virus (HBV) genotype based on suspension chip. Methods Based on the DNA sequence information of 8 HBV genotypes, the related primers and probes were designed and synthesized. The PCR products were amplified by PCR. The fluorescence signals were detected after hybridization with the nucleic acid probe microspheres. Results The sensitivity of the suspension chip method for detection of HBV-A, HBV-E and HBV-G was 9 DNA copies. The sensitivity of HBV-B, HBV-C, HBV-D, . Compared with the fluorescence PCR method, the difference was not statistically significant. Conclusion The detection method of suspension chip which can detect 8 HBV genotypes at the same time is established, which provides a new method for rapid screening and identification of HBV.